Daejeon Health Sciences College Museum was established as a subsidiary museum of the College after having enacted the Museum Regulations on March 1, 1992 and acquired approval for establishment in accordance with the College Regulations on May 14, 1992.

In the initial stage of the establishment, simple geological survey of the historical remains in the region of Daejeon was executed with the Literary Research Center playing the key role. That is, beginning with the geological surveying of the group of ancient tombs in Jusan-dong, Dong-gu, Daejeon City, cluster of ancient tombs of the Era of the Three States scattered in the submerged regions of Unmun Dam in Cheongdo-gu, Gyeongsangbuk-do was excavated and examined over the period of September 1, 1993 to January 11, 1994. With the completion of the construction for the main building of the Museum in November 1994, Office of the Director of the Museum, Literary Research Center, Collection Warehouse, Material Organization Room and Exhibition Hall was established on the 3rd floor of the newly constructed building in March 1995. With the expansion of the exhibition space, the Museum was able to continuously collect more extensive materials through activities such as surveying of the prehistoric remains in Gwanchang-ri, Boryeong City, Chungnam, cultural remains in Songchond-ong, Daejeon and the site of kiln for production of roof tiles in Gwanhyeon-ri, Cheongyang, etc. The teaching staffs of the College donated medical devices, school magazines and newsletters, and folklore materials, etc. while the administration department donated the office fixtures used at the time of the founding of the College. Accordingly, the Museum is currently preserving and managing historical materials along with school history materials.

Following the opening of the exhibition hall to the public on October 18, 1998, the Museum concentrated on the collection of materials and their exhibitions along with publication of report on the excavation survey on site of Sunghyeon Seowon (lecture hall) (2002), report on the excavation survey of the remains in Gwanchang-ri, Boryeong (2002) and report on the excavation survey of the remains in Songchon-dong, Daejeon (2002), etc. in addition to simple geological surveys for 10 years thereafter. The College joined as a member school of the Korean Association of University Museums on October 16, 1998 and was registered as a Category 1 museum (specialized museum) with the Ministry of Culture and Tourism on January 26, 2001. In August 2007, the Museum joined the Association of Museums of Daejeon Metropolitan City as a member.